- Squirting bacteriostatic water straight onto the lyophilised cake at speed.
- Shaking the vial vigorously to dissolve — use gentle swirl only.
- Reusing needles between vials without a documented change protocol.
- Leaving reconstituted material at room temperature during a long bench session.
- Assuming “more BAC = safer” without recalculating concentration in the calculator.
- Using plain sterile water when your SOP calls for bacteriostatic water (different stability profile).
- Run BAC down the glass wall slowly; wait for full dissolution before use.
- Use the reconstitution calculator for U-100 unit arithmetic — never eyeball draw volume.
- Verify batch and log reconstitution date in your lab notebook or LIMS.
- Discard clouded or particulate solutions per institutional waste policy.
If a vial fails to dissolve after extended gentle mixing, do not heat unless your validated SOP explicitly allows it — contact verify@tararesearch.ie with batch number for QA review.